Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience

Beside the training of different sample preparation techniques some scientists brought their own samples for dissection. One task was to collect the whole substantia nigra from an unstained mouse brain (Fig. 2). “After the evaluation of some other systems the participants were surprised by the power of the Leica LMD system,” Falk said. “Our laser allows to dissect the 70 µm unstained section with a single cut, by the speed and option to collect big areas covering multiple fields of view. In this way, it was possible to collect the desired brain area from all provided samples in very little time. Such large laser microdissected areas of unstained and well-defined brain layers are perfect starting material for proteomics and metabolomics.” The second task was to dissect individual cells – instead…


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