PhD-student in cellular neuroscience (electron microscopy, super resolution imaging)

Functieomschrijving Our lab has identified core components of the docking machinery and how they work together to dock secretory vesicles . However, how the docking machinery is positioned at the appropriate site for secretion, for instance in the active zone of the nerve terminal, remains unknown. In this project, we aim to investigate the link between the docking machinery and (a) the sub-membrane cytoskeleton and (b) lipid micro-domains in the plasma membrane that can affect the positioning of the secretion site. You will investigate alterations in cytoskeleton, lipid micro-domains and neurotransmitter vesicle docking using cryo/3D electron microscopy, super resolution imaging (structural illumination/dSTORM) and functional assays (e.g. fluorescence vesicle fusion assays, electrophysiology) in neurons and neuroendocrine cells of available genetic mouse models. Novel links between the docking machinery and the cytoskeleton/lipid…


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