Novel transcripts reveal a complex structure of the human TRKA gene and imply the …

An elaborate arrangement of the human TRKA gene revealed by novel transcription initiation sites In silico analysis of the human TRKA gene structure using UCSC genome browser [42] to align the TRKA mRNAs and expressed sequence tags (ESTs) from GenBank to the genomic sequence indicated a higher level of variability among TRKA transcripts and a longer span of the gene than previously described in the literature. For this reason, we performed reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of 5′ complementary DNA ends (5′ RACE) analyses to better describe TRKA gene and its transcripts. In the RT-PCR study, we used a selection of adult and fetal tissues and different regions of the nervous system. Also included were neuroblastoma cell-lines SH-SY-5Y and SK-NMC which are known to express TRKA.…


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