July 2016

David I. Wasserman, Joel M. J. Tan, Jun Chul Kim and John S. Yeomans To inhibit or excite RMTg GABA neurons, M4D or M3D DREADDs were utilized in GAD2::Cre mice. While no effect was observed with vehicle-induced locomotion (top panels), M4D inhibition of RMTg GABA neurons increased, and M3D excitation blocked, morphine-induced locomotion (bottom panels). M4 muscarinic receptors were found colocalized with 84% of μ-OR-positive RMTg GABA neurons. This suggests that muscarinic input to RMTg facilitates morphine-induced locomotion due to inhibition.

Link to Full Article: July 2016