Detecting false positive sequence homology: a machine learning approach

Library preparation and RNA-seq For the experimental data set (OD_S) we used 18 Odonata (dragonflies and damselflies) and 2 Ephemeroptera (mayflies) species. Total RNA was extracted from the eye tissues of each taxon using NucleoSpin RNA II columns (Clontech) and reverse-transcribed into cDNA libraries using the Illumina TruSeq RNA v2 sample preparation kit that both generates and amplifies full-length cDNAs. Prepped Ephemeroptera mRNA libraries were sequenced on an Illumina HiSeq 2000 producing 101 bp paired-end reads by the Microarray and Genomic Analysis Core Facility at the Huntsman Cancer Institute at the University of Utah, Salt Lake City, UT, USA, while all Odonata preps were sequenced on a GAIIx producing 72 bp paired-end reads by the DNA sequencing center at Brigham Young University, Provo, UT, USA. The expected insert sizes were 150 bp and…


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