Deletion of JMJD2B in neurons leads to defective spine maturation, hyperactive behavior and …

Top of page Materials and methods Mice For all experiments, the mice were of the C57B6/J strain. Jmjd2b floxed mice, in which exon 5 of the Jmjd2b allele was flanked by loxP sequences bilaterally,13 were crossed with mice expressing Cre recombinase under the control of the Tau promoter,21 resulting in the specific deletion of JMJD2B in neurons. Both genders were used in all experiments except for behavioral analysis (male mice). In all cases, pups were weaned on postnatal day (P) 28, and housed in sex-matched groups in standard mouse cages under a 12-h light/dark cycle according to the protocols approved by Institutional animal care and Use Committee of Osaka University. In situ hybridization Mice were harvested for in situ hybridization analysis at embryonic days (E) 13, 15 and 17, and…


Link to Full Article: Deletion of JMJD2B in neurons leads to defective spine maturation, hyperactive behavior and …