An integrative approach for analyzing hundreds of neurons in task performing mice using wide …

Wide-field imaging of hippocampal CA1 neurons in awake behaving mice To demonstrate the use of wide-field microscopy across large brain areas at high spatial and temporal resolution we constructed a conventional epifluorescence microscope for recording in awake head fixed mice. The microscope consists of a 10X objective lens for increased imaging area, a high-intensity LED that can be precisely controlled via TTL pulses, a filter set appropriate for imaging GCaMP6 fluorescence, and a scientific CMOS camera capable of imaging large areas at high speed (Fig. 1A). In order to achieve high image quality with good signal-to-noise ratio, we imaged the hippocampal CA1 region; the CA1 region consists of a thin and densely packed pyramidal cell layer nested between two thick and sparsely populated layers (Fig. 1B). This unique anatomical organization…


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